The instruments belonging to the JASCO FP-8050 spectrofluorometer family introduced in 2021, in accordance with the JASCO traditions, are double monochromator instruments, in which both the excitation and the emitted light are resolved by separate monochromators. Due to the precise optical system, the spectrofluorometers have excellent signal-to-noise ratio, resolution, wavelength accuracy and scattered light values. The instruments are characterized by user-friendly solutions, such as automatic sample holder recognition and the Start button placed on the top of the instrument, which allows automatic sample recognition or immediate measurement after the sample placement. Due to the innovations, the continuous-output xenon lamp has a lifespan three times longer than before.
The spectrofluorometers are controlled by the Spectra Manager software, which controls the JASCO spectroscopic instruments and has a rich selection of standard measuring and evaluation programs. As a result of all the features mentioned above, the FP-8050 fluorometers are just as well-suited in quality control laboratories that perform routine measurements as they are in Life Science or Materials Science research laboratories.
JASCO FP-8250 spectrofluorometer
Developed primarily for routine measurements, the FP-8250 features new developments that allow high-performance measurements. It has a wide dynamic range, high resolution within its category and a built-in mercury lamp for wavelength accuracy control. Due to these features, it performs well in university, research, GLP and pharmaceutical environments as well. The simple but robust design allows fluorescence (with excitation, emission, and synchronous measurement modes) and chemiluminescence, and with suitable optional sample holders allows single-drop, transmittance, absorbance, fluorescent polarization, kinetics and temperature-dependent measurements.
Specifications:
- Wavelength range:
- Excitation (Ex): 200-750 nm
- Emission (Em): 200-750 nm
- Monochromators: 1650 lines/mm
- Selectable spectral bandwidth: 1, 2.5, 5, 10 or 20 nm
- Wavelength accuracy: ± 1.5 nm
- Selectable scanning speed: 20 – 20,000 nm/min
- Signal-to-noise ratio (RMS) (at water Raman peak, Ex and Em 5 nm bandwidth, response: 2s) better than 680:1 (noise at ~ 350 nm); better than 4500:1 (noise at 450 nm).
- Auto-gain and auto-sensitivity adjustment
- Built-in mercury lamp for wavelength accuracy control
- Gas inlet to purge the sample compartment
- Start button, analog signal output and automatic accessory recognition system
- Control: Spectra Manager 2.5 software
JASCO FP-8350 spectrofluorometer
A spectrofluorometer developed to meet the needs of Life Science laboratories. Nearly 50 different sample holders are available to make the measurement tasks as easy as possible during research. These tasks include the sample holders that are temperature-controlled with the water- or Peltier-type thermostats, microcuvette holders for measuring microliter samples, and “one-drop” accessories capable of testing the fluorescence of droplets. For the measurement of a large number of samples autosampler and flow cells are available. For the direct measurement of samples stored in microplates a microplate reader can be connected to the spectrofluorometer. For the kinetic and reaction analyzes there are a lot of possibilities available: from the sample compartment lid with injection facilities, through the automatic titration instrument to the “stopped-flow” supplements. For phosphorescence measurements nitrogen-cooled liquid and solid sample holders can be used, as well.
Specifications:
- Wavelength range:
- Excitation (Ex): 200-750 nm
- Emission (Em): 200-750 nm, with optional PMT detector: 200-900 nm. Monochromator: 1650 lines/mm
- Monochromators: 1650 lines/mm
- Selectable spectral bandwidth: 1, 2.5, 5, 10 or 20 nm
- Wavelength accuracy: ± 1.5 nm
- Selectable scanning speed: 20 – 20,000 nm/min
- Signal-to-noise ratio (RMS) at water Raman peak, Ex and Em 5 nm bandwidth, response: 2s) better than 880:1 (noise at ~ 350 nm); better than 8000:1 (noise at 450 nm).
- Auto-gain and auto-sensitivity adjustment
- Built-in mercury lamp for wavelength accuracy control
- Automatic cut filters for higher order diffraction
- Start button, analog signal output and automatic accessory recognition system
- Control: Spectra Manager 2.5 software
JASCO FP-8550 and FP-8650 spectrofluorometers
The FP-8550 with its extremely high signal-to-noise ratio and the FP-8650 with its wavelength range extended to 1010 nm can be of great interest particularly for the materials science laboratory workers. As sample holders, beside the ones mentioned at the FP-8350 used for solid sample measurements, the different film holders, manual and automatic polarizers, accessories for measuring epifluorescence and integrating sphere of 60 mm and cooled integrating sphere of 120 mm diameter capable of measuring the quantum efficiency must be mentioned.
Specifications FP-8550:
- Wavelength range:
- Excitation (Ex): 200-850 nm
- Emission (Em): 200-850 nm
- Monochromators: 1800 lines/mm
- Selectable spectral bandwidth: 1, 2.5, 5, 10 or 20 nm, special bandwidth adjustment for small sample volumes
- Wavelength accuracy: ± 1.0 nm
- Selectable scanning speed: 10 – 60000 nm/min (Ex and Em)
- Signal-to-noise ratio (RMS) (at water Raman peak, Ex and Em 5 nm bandwidth, response: 2s) better than 1400:1 (noise at ~ 350 nm); better than 8500:1 (noise at 450 nm).
- Auto-gain and auto-sensitivity adjustment
- Built-in mercury lamp for wavelength accuracy control
- Automatic cut filters for higher order diffraction
- Start button, analog signal output and automatic accessory recognition system
- Control: Spectra Manager 2.5 software
Spectra Manager 2.5 for spectrofluorometers
For the control and data processing of spectrofluorometers even the standard package of Spectra Manager 2.5 contains the programs necessary for excitation (Ex) and emission (Em) spectra acquisitions, for simple kinetic measurements, quantitative analysis and fixed wavelength measurements. For the FP-8350, 8550 and 8650 instruments it also contains the phosphorescence measurement modes. The standard software package includes a 3-dimensional spectra measurement program used for finding the optimal excitation and emission wavelengths manually or automatically, as well as modules that make possible for the user to validate the instrument and to check it daily.
To meet the specific needs of various disciplines, there is a choice of a large number of optional softwares which include, among others, the program performing kinetic measurements at two wavelengths for intracellular calcium measurement, the fluorescence color definition or the quantum efficiency measurement module, as well.
The standard software package includes:
- Quantitative analysis with calibration curve
- Spectral scan recording on Ex and Em sides
- 3-dimensional data collection
- Simple kinetics
- Parallel kinetics measurement with multi-position cuvette changers
- Daily check
- Validation
- Instrument activity log
- Instrument diagnostics
- Spectrum analysis, derivatives and other mathematical functions, peak search, “overlay”, report and style formatting
Optional softwares:
- Temperature interval scan measurement program
- DNA melting point calculation
- Dual wavelength time course measurement program
- Macro command program – allows creation of macros by the user
- Fluorescence polarisation measurement
- Fluorescence color measurement program
- Luminant color measurement program
- Advanced kinetic analysis program
- Quantum yield calculation program
Sample holders
The standard cell holder of the JASCO FP-8050 spectrofluorometers are able to hold cuvettes of 10 x 10 mm. Optionally, water- and Peltier thermostatted cuvette holders, micro cuvette holders, sample holders capable of measuring solids, stopped-flow systems and microplate readers can be attached to the instruments.
Optional sample holders:
- Water thermostattable cell holder with magnetic stirrer
- Peltier thermostatted single cell holder with magnetic stirrer, temperature control range 0 – +100 oC
- Peltier thermostatted 4- and water termostatted 8-position cuvette holders with magnetic stirrer
- Micro cuvette holders
- “One drop” accessory for droplet measurement
- Attachment for absorbance measurement
- 30o incident angle cell holder
- Film holder and powder sample cell block
- Epifluorescence attachment
- Manual and automated polarizer accessory
- Automatic titration accessory with two syringes
- Vacuum and peristaltic pump sippers
- Autosampler
- Peltier thermostatted „Stopped-flow” system
- Microplate reader attachment for 96- and 384-well microplates.
- Low temperature measurement attachment for phosphorescence and fluorescence measurements
- High temperature accessory for powders
- Integrating spheres for quantum yield determination and luminous color measurement
