Spectroscopy

V-700 UV/VIS/NIR  spectrophotometers image

V-700 UV/VIS/NIR spectrophotometers

All members of the JASCO V-700 double beam spectrophotometer family own the following characteristics: a massive, robust construction, precision and excellent optical parameters. During the creation of the instruments it was important that a user-centered design should be developed. A good example for this aspiration is that each device is equipped with a Start button and after placing the sample into the sample holder the measurement can be started immediately. Due to the automatic accessory recognition system, when changing the sample holders, the setup options of the newly inserted sample holders appear in the software instantly.

The smallest V-730 is primarily used for routine measurements and has an important role in applied research work; V-750 can be used in research, industry and education; the V-760 with its double monochromator optical system makes possible the measurement of samples with extremely high absorbance without dilution; the V-770 due to its extended wavelength range to near-infrared region (NIR) shall become an essential instrument of spectroscopic laboratories.

With the V-700 spectrophotometers more than 50 different types of sample holders can be used: including the accessories for the measurement of droplet volume samples, the thermostattable multi-positioned sample holders, integrating spheres that allow the measurement of textiles or the measurement of the diffuse reflectance of powders. With these accessories almost all spectroscopic measurements can be carried out. Their control and collection of the measured data is provided by the integrated Spectra Manager II Windows-based software available for the pharmaceutical industry also in the 21CFR Part 11 compatible version. The software is able to control all the JASCO spectroscopy instruments.

JASCO V-730 spectrophotometer

This is a double beam UV-Vis spectrophotometer with a fixed 1 nm bandwidth ideal for control laboratories that carry out a large number of routine measurements. Due to its optical specifications considered excellent within its category and its optionally large number of sample holders it can be used for industrial, pharmaceutical or even biochemical or other “Life Science” discipline measurements, as well. With its optional thermostattable sample holders, the kinetic measurements can be solved quickly and easily.

This spectrophotometer is controlled either with the Spectra Manager II software or with the handheld iRM (intelligent Remote Module) control unit. The well-organised software environment of iRM includes all programs necessary for instrument control, data acquisition and data processing. With the help of these programs and the directly connected printer the measurements can be carried out without a computer with the possibility of documentation.

Specifications:

  • wavelength range: 190-1100 nm
  • light source: deuterium and halogen lamp
  • detector: photodiode
  • spectral bandwidth: 1 nm
  • wavelength change speed: 24000 nm/min
  • scanning speed: 10 – 8000 nm/min
  • wavelength accuracy: ±0.2 nm
  • wavelength reproducibility: ±0.1 nm
  • photometric range: -3 – +3 AU
  • photometric accuracy: ±0.0015 AU (0-0.5 AU)
  • photometric reproducibility: ±0.0005 AU (0-0.5 AU)
  • stray light at 220 nm: 0.02%
  • RMS noise: 0.00004 AU (0 AU, 500 nm)
  • baseline stability: ± 0.0004 AU/hour
  • dimensions and weight: 486(L)x441(W)x216(H) mm, 15 kg
  • control possibilities: PC software or standalone iRM module with graphical interface

JASCO V-730BIO spectrophotometer

This is a double beam UV-Vis spectrophotometer with a fixed 1 nm bandwidth operated with an iRM controller that has a software package compiled for ”Life Science” applications. The use of a computer is not required, thus its little space allowances, its sample holders capable of measuring jasco_v730bio_irmmicroliters of samples and its excellent optical properties allow for the determination of proteins and nucleic acids or kinetic measurements in science laboratories of biochemistry, biotechnology, and of other related fields. Due to the easy handling, the accessory detection system and the one-button measurement, everyday routine measurements can be carried out easily within a short time.

Beside the standard measurement methods, the control unit contains the software necessary for determining the protein/nucleic acid ratio (A260/A280, A230/A260, Warburg-Christian), the kinetic analysis program (according to Michaelis-Menten, Lineweaver-Burk, Hofstee and Eadie methods), a software for quantitative determination of proteins, performing calculations based on UV absorption, BCA, Bradford, Lowry and Biuret methods and a software for testing the DNA melting.

Specifications:

  • wavelength range: 190-1100 nm
  • light source: deuterium and halogen lamp
  • detector: photodiode
  • spectral bandwidth: 1 nm
  • wavelength change speed: 24000 nm/min
  • scanning speed: 10 – 8000 nm/min
  • wavelength accuracy: ±0.2 nm
  • wavelength reproducibility: ±0.1 nm
  • photometric range: -3 – +3 AU
  • photometric accuracy: ±0.0015 AU (0-0.5 AU)
  • photometric reproducibility: ±0.0005 AU (0-0.5 AU)
  • stray light at 220 nm: 0.02%
  • RMS noise: 0.00004 AU (0 AU, 500 nm)
  • baseline stability: ± 0.0004 AU/hour
  • dimensions and weight: 486(L)x441(W)x216(H) mm, 15 kg
  • standard cuvette holder: with the cell height adjustment function
  • control possibilities: standalone iRM module with touchscreen

 JASCO V-750 spectrophotometer

This is a double beam UV-Vis spectrophotometer with adjustable bandwidth. Due to its highly sensitive photomultiplier (PMT) detector, it can be used for research and development purposes as well as for routine measurements of high precision. In the selection of optional sample holders, beside the integrating spheres of 60 and 150 mm used for diffuse reflectivity measurements of solid samples also accessories for the determination of absolute reflection can be found. With the film holder rotatable in all directions film thickness and transmittance of solid samples, glass and filters can be examined.

Specifications:

  • wavelength range: 190-900 nm
  • spectral bandwidth: variable between 0.1-10 nm, dedicated slit shape for micro cuvettes
  • light source: deuterium and halogen lamp
  • detector: photomultiplier (PMT)
  • wavelength change speed: 12000 nm/min
  • scanning speed: 10 – 4000 nm/min, in preview mode 8000 nm/min
  • wavelength accuracy: ±0.2 nm
  • wavelength reproducibility: ±0.05 nm
  • photometric range: -4 – +4 AU
  • photometric accuracy: ±0.0015 AU (0-0.5 AU)
  • photometric reproducibility: ±0.0005 AU (0-0.5 AU)
  • stray light at 220 nm: 0.005%
  • RMS noise: 0.00003 AU (0 AU, 500 nm)
  • baseline stability: ± 0.0003 AU/hour
  • dimensions and weight: 460(L)x602(W)x268(H) mm, 27 kg
  • control possibilities: with SpectraManager II PC software

JASCO V-760 spectrophotometer

 This double beam UV-Vis spectrophotometer with adjustable bandwidth containing a double monochromator is capable of measuring in the wavelength range between 187-900 nm. Due to its very low stray light value, samples with even 6AU absorbance value can be measured without dilution. It has a wide range of absorbance, which allows for spectroscopy research or characterisation of optical filters and standards, as well.

 

Specifications:

  • wavelength range: 187-900 nm
  • spectral bandwidth: variable between 0.1-10 nm, dedicated slit shape for micro cuvettes
  • light source: deuterium and halogen lamp
  • detector: photomultiplier (PMT)
  • wavelength change speed: 12000 nm/min
  • scanning speed: 10 – 4000 nm/min, in preview mode 8000 nm/min
  • wavelength accuracy: ±0.1 nm
  • wavelength reproducibility: ±0.05 nm
  • photometric range: -4 – +6 AU
  • photometric accuracy: ±0.0015 AU (0-0.5 AU)
  • photometric reproducibility: ±0.0005 AU (0-0.5 AU)
  • stray light at 220 nm: 0.00008%
  • RMS noise: 0.00003 AU (0 AU, 500 nm)
  • baseline stability: ± 0.0003 AU/hour
  • dimensions: 460(L)x602(W)x270(H) mm, 29 kg
  • control possibilities: with SpectraManager II PC software

JASCO V-770 spectrophotometer

This is a double beam UV-Vis/NIR spectrophotometer with adjustable bandwidth capable of measuring not only in the ultraviolet and visible range but also in the near infrared (NIR) region. The measurement in the NIR region is possible with a separate grid and a Peltier-cooled PbS detector. The basic construction of the instrument can be used for measurements between 190-2700 nm, optionally between 190-3200 nm, thus it can be used for quality control and development of different optical elements, as well.

Specifications:

  • wavelength range: 190-2700 nm, optionally expansible: 190-3200 nm
  • light source: deuterium and halogen lamp
  • scanning speed: 10 – 4000 nm/min, in preview mode 8000 nm/min
  • photometric accuracy: ±0.0015 AU (0-0.5 AU)
  • photometric reproducibility: ±0.0005 AU (0-0.5 AU)
  • RMS noise: 0.00003 AU (0 AU, 500 nm)
  • baseline stability: ± 0.0003 AU/hour

UV-VIS specifications:

  • spectral bandwidth: variable between 0.1-10 nm, dedicated slit shape for micro cuvettes
  • detector: photomultiplier (PMT)
  • wavelength change speed: 12000 nm/min
  • wavelength accuracy: ±0.3 nm
  • wavelength reproducibility: ±0.05 nm
  • photometric range: -4 – +4 AU
  • stray light at 220 nm: 0.005%

NIR specifications:

  • spectral bandwidth: variable between 0.4 – 40 nm, dedicated slit shape for micro cuvettes
  • detector: Peltier cooled PbS.
  • wavelength change speed: 48000 nm/min
  • wavelength accuracy: ±1.5 nm
  • wavelength reproducibility: ±0.2 nm
  • photometric range: -3 – +3 AU
  • stray light at 1690 nm: 0.1%

 

  • dimensions: 460(L)x602(W)x268(H) mm, 29 kg
  • control possibilities: with SpectraManager II PC software

JASCO V-780 spectrophotometer

Beside the measurements within the ultraviolet and visible ranges, with the JASCO V-780 UV-Vis/NIR spectrophotometer measurements of high sensitivity and high resolution can be carried out within the near infrared range as well.

JASCO_V780_spektrum
JASCO V-780 with absolute reflectance accessory

The measurement in the NIR range is possible with a separate grid (600 lines/mm) and a Peltier-cooled InGaAs photodiode detector. Due to its measuring range between 190-1600 nm this instrument is ideal for quality control and development of optical network components, polarizers, prisms, lenses, optical fibres, notch-, edge- and band-pass filters etc. The optional sample holders available in a large number can help the measurement of the samples of various shapes, sizes and optical properties. Thus, the measurement of the anti-reflective (AR) coatings within the wavelength range between 1200-1500 nm becomes possible, as well as the examination of the polarizators crossed Nicols state.

Specifications:

  • wavelength range: 190-1600 nm
  • light source: deuterium and halogen lamp
  • scanning speed: 10 – 4000 nm/min, in preview mode 8000 nm/min
  • photometric accuracy: ±0.0015 AU (0-0.5 AU)
  • photometric reproducibility: ±0.0005 AU (0-0.5 AU)
  • RMS noise: 0.00003 AU (0 AU, 500 nm)
  • baseline stability: ± 0.0003 AU/hour

UV-VIS specifications:

  • spectral bandwidth: variable between 0.1-10 nm, dedicated slit shape for micro cuvette
  • detector: photomultiplier (PMT)
  • wavelength change speed: 12000 nm/min
  • wavelength accuracy: ±0.3 nm
  • wavelength reproducibility: ±0.05 nm
  • photometric range: -4 – +4 AU
  • stray light at 220 nm: 0.005%

NIR specifications:

  • spectral bandwidth: variable between 0.2 – 20 nm, dedicated slit shape for micro cuvettes
  • detector: Peltier-cooled InGaAs photodiode
  • wavelength change speed: 24000 nm/min
  • wavelength accuracy: ±1.0 nm
  • wavelength reproducibility: ±0.1 nm
  • photometric range: -3 – +3 AU
  • stray light at 1420 nm: 0.04%

dimensions: 460(L)x602(W)x268(H) mm, 29 kg
control possibilities: with SpectraManager II PC software

Spectra Manager II software package for V-700 spectrophotometers

The Spectra Manager II used for V-700 spectrophotometers in its basic package contains an ample selection of programs for measurement and evaluation. However, if the task requires, there is a choice of more than 20 other optional softwares from which the most suitable one can be chosen.

- measurement modes:

  • absorbance and transmittance
  • quantitative analysis with calibration curve
  • spectrum scanning
  • fixed wavelength mode on several wavelengths
  • simple kinetic measurement

– device test:

  • autodiagnostics
  • wavelength calibration
  • automatic accessory detection and registration (GLP compliance)

– functions:

  • validation software with USP, EP and JP compliance module, with possibility of user defined setup
  • data storage and search function
  • statistical analysis of calibrating curve with tolerance ranges
  • spectrum analysis: peak search, matematical functions, derivatives, baseline correction, conversions, overlay
  • color analysis
  • simple enzyme kinetics
  • film thickness measurement
  • report templates with various styles

Software options:

  • Temperature gradient program package for measuring the temperature dependence of transmittance/absorbance/reflectance at given wavelengths
  • Time interval scan – measures spectra automatically at preselected time intervals using the preprogrammed parameters. Includes the Interval Data Analysis module to handle 3-dimensional data.
  • Temperature interval scan – measures spectra automatically at preselected temperatures using an optional Peltier thermostatted cell holder. Includes the Interval Data Analysis module to handle 3-dimensional data.
  • Kinetic analysis program – allows calculation of initial speed of enzyme reactions, maximal speed, Michaelis-Menten constant, inhibition constant and type of inhibition. 5 different reaction kinetic models are available for selection.
  • Protein/Nucleic acid determinations – offers a choice different calculation methods based on dual wavelength absorbance measurements among others the generally recognized Warburg-Christian method. Allows creation of user defined methods.
  • PLS (Partial Least Squares), CLS (Classical Least Squares), PCR (Principal Components Regression) and PCA (Principal Components Analysis) Quantitative programs for analysis of multicomponent samples based on spectral data
  • Spectral quantitative analysis program „Band gap” analysis program for energy calculations from transmission and reflexion spectra of semiconductors
  • Color diagnosis program with possibilities of color calculation, color comparison, color matching and color difference in various color system
  • Luminous color measurement program for color and color matching analysis of light emitting sources (e.g. LED); (external light source interface and calibrated light source is needed for measurement)
  • Color matching program. With possibility to create color library from standard samples; with color calculation and color comparison based on the spectrum of the target sample
  • Saybolt and ASTM color analysis programs for petrochemical products
  • Chromaticity/turbidity measurement program for water purity analysis
  • Determination of UV shielding factor – calculates the UV shielding factor corresponding to predetermined wavelength ranges, based on measured spectra
  • Solar/visible light measurement program for calculation of solar light transmittance/reflectance and visible light transmittance/reflectance/ absorbance based on standard methods
  • Haze measurement program for analysis of plastic films, glass samples with pass/fail (conformance) function
  • SPF/PA calculation program for determination of UV-B/UV-A protection grade of cosmetics, clothing and sunglasses
  • Spectral concatenation program
  • Reflectance correction program for specular reflectance accessories
  • „Macro Command” program – allows creation of macros by the user for data measurement, handling, presentation, sorting into worksheet etc. Possibility to define, compare and calculate with variables.
  • Data conversion program – performs conversion of data stored in .jws (Ver.2.0) format and the directly non-compatible .jws (Ver. 1.5) as well as JCAMP-DX and .txt formats in both directions.

» manufacturer's page »

 

The standard cell compartment of the JASCO V-700 spectrophotometer possesses a sample and reference cuvette holder capable of receiving 10×10 mm cuvettes. There are also available optionally manual and automatic cell changers, cuvette holders for the long cuvettes, water thermostattable cuvette holders and cell changers.

  • Cell holder for 10, 20, 50 and 100 mm path rectangular cuvettes
  • 4 and 6 position manual and automated cell holders for long path rectangular cuvettes
  • cylindrical cell holder for analysis of gases and liquids (for cylindrical cuvettes with maximum 100 mm long and with outer diameter 22 or 30 mm)
  • water thermostattable on both sample and reference sides cuvette holder also for 10 mm cuvettes and microcuvettes
  • water thermostattable manual and automatic cell changers with 4 or 6 positions on sample side, and one reference position, for 10 mm cuvettes
  • sample compartment lid with syringe port on sample side

The Peltier thermostated cuvette holders are useful for measuring solutions at the controlled temperature, for studying the spectral changes depending on the temperature changes. The temperature setting takes place in the Spectra Manager II software (or on the IRM controller). Each Peltier thermostated JASCO cuvette holder has a magnetic stirrer whose speed can be controlled by a software. With the optional temperature sensors even the temperature of the solution can be measured directly.

  • Cell holder with Peltier-thermostatting on sample side only, with magnetic stirrer, temperature range: 10-60 °C or 0-100 °C
  • Cell holder with Peltier-thermostatting on sample and reference sides, water cooled, with magnetic stirrer, temperature range: 0-100 °C
  • 6 or 8 position automatic cell changer with Peltier- thermostatting on sample side only, with magnetic stirrer, one reference position, temperature range: 15-60 °C or 0-100°C
  • 6 or 8 position automatic cell changer with Peltier-thermostatting on sample positions and on single reference position, water cooled, with replaceable cell holder block, with magnetic stirrer, temperature range: 0-100 °C

For the measurement of biological and biochemical samples, micro cell holders and microcuvette holders are available, which allow the measurement of micro-liter quantities easily, accurately and reproducibly. In the supply there are holder capable of measuring with only one droplet, as well as the capillary cell holder mounted with input/output lenses and the 8-position, rotatable micro cell holder.

  • cell holder with sample side cell height setting (for cuvettes with 10 mm light path) and with removable sample side 1,5×3 mm (height) lightpath mask
  • micro cell holder with cell height setting for 50-5 µl volume
  • „One Drop” microsampling accessory for measuring microvolume samples. Minimum sample volume with 1 mm pathlength disk 5 µl, with 0.2 mm pathlength disk 0,6 µl. Easily to demount and to clean between samples
  • Capillary cell adaptor with input/output lenses, accomodated in place of a 10×10 mm cuvette. Easily changeable quartz capillaries of abt. 0.5 mm optical pathlength, minimum sample requirement is 3 µl.
  • 8-position rotatable micro cell holder, light path 1 mm, sample volume 4 µl
  • Wash special pipette for cleaning micro cells

For measuring a large number of samples, in the supply there are available vacuum and peristaltic sippers, different types of optical path length flow cells, and auto samplers allowing full automation.

  • vacuum sipper with flow through cell with 10 mm or 50 mm lightpath
  • peristaltic sipper with flow through cell with 10 mm lightpath (cell volume 50 µl, required sample volume 0.7 ml)
  • cell holder for 5 and 10 mm or 30 – 100 mm lightpath flow through cells
  • autosampler for 15×105, 13×100, 12×105, 10×90 mm test tubes and for HPLC vials with slit septa

The integrating spheres of 60 mm or 150 mm internal diameters, which allow diffuse reflectance and transmittance measurements and are used with the JASCO V-750/760/770 instruments, are indispensable to measurements of powders, textiles, packaging materials, and other solids or cloudy liquids normally not capable of spectrophotometric measurement under normal conditions.

  • 60 mm and 150 mm integrating spheres with built-in PMT detector for UV-Vis and built-in PbS detector for NIR region
  • 60 mm portable integrating spheres with built-in PMT detector for UV-Vis and built-in PbS detector for NIR region
  • 60 mm integrating spheres with horizontal sample surface with built-in PMT detector for UV-Vis and built-in PbS detector for NIR region
  • 60 mm integrating spheres with magnetic stirrer with built-in PMT detector for UV-Vis and built-in PbS detector for NIR region
  • integrating spheres for special purposes (e.g. gem examination)

A large number of further sample holders are available for specular reflectance, absolute reflectance and the various incident angle measurements of films. For special tasks fiber optic adapters are also available.

  • specular reflectance accessory
  • Manually adjustable absolute reflectance measurement accessory with built-in 60 mm integrating sphere for synchronous and asynchronous measurement modes, for measuring absolute and relative reflexion and diffuse transmission
  • Automatic absolute reflectance measurement accessory with built-in 60 mm integrating sphere for asynchronous measurement mode for measuring absolute and relative reflexion as well as diffuse transmission with variable incident angle

Software options for absolute reflectance measurement accessory:

  • Variable incident angle measurement program
  • Average reflectance calculation program
  • Multilayer film thickness analysis program
  • Phase different measurement program

Film holders

  • film holder with spring; film thickness: 0.5-10 mm, maximum sample size: 80×100 mm, minimum sample size: 15×15 mm
  • film holder, sliding mount type, film thickness 0.5-25 mm, maximum sample size: 80×100 mm, minimum sample size: 5×5 mm
  • film holder with adjustable incident angle; film thickness: 1-2 mm, maximum sample size: 80×70 mm, minimum sample size: 15×35 mm, rotation range: ±90o
  • film holder with adjustable incident angle, the planar rotation of sample is possible in 360o, film thickness: 1-2 mm, maximum sample size 18×38 mm, minimum sample size: 10×30 mm, the rotation of incident angle is possible in range of ±50o

Fiber optic accessories:

  • Fiber optic immersion probe with interface and optic cable, 10 mm optical pathlength; usable wavelength range ~220-2300 nm; outside diameter 20 mm, total length 180 mm, max. immersion depth 100 mm, max. pressure 6 bar, max. temperature 150°C; fiber optic cable interface; changeable 2 pcs 2 m optic cable