Spectroscopy

Hellma cuvettes image

Hellma cuvettes

The German Hellma Group founded in 1922 provides continuously special optical accessories at the highest level for those operating in the most varied fields of science and industry. Hellma Analytics, one of the members of the group, produces glass and quartz cuvettes, optical calibration standards, optic probes and optic fibers create for the users.

The material of the cuvettes determines the wavelength range in which the qualitative and quantitative measurements are recommended.
Material Marker Wavelength[nm]
optical glass OG 360 – 2500
special optical glass OS 320 – 2500
Quartz Suprasil QS 200 – 2500
Material Optical path length [mm] Tolerance [mm]
quartz 0.01 – 0.05 ± 0.003
quartz 0.1 – 0.2 ± 0.005
quartz 0.5 – 20 ± 0.01
quartz 30 – 100 ± 0.02
special optical glass 0.1- 20 ± 0.01
special optical glass 30 – 100 ± 0.02
optical glass 10 – 30 ± 0.1
optical glass 40 – 100 ± 0.2
The optical path length is also an extremely important parameter of the cuvettes. In the table the optical path length tolerances related to the absorption cuvettes are shown. The path length tolerance of the fluorescence cuvettes on the excitation and emission side is ±0.05 mm.

Below we provide a selection of the most common cuvette types and cuvettes. If you cannot find on our website the product you are looking for, please, do not hesitate to contact us.

Glass cuvettes Special optical glass cuvettes Quartz cuvettes Semi-micro and micro cuvettes
Cuvettes for magnetic stirrers Fluorescence cuvettes Cylindrical cuvettes  Flow-through cuvettes
Demountable cuvettes Calibration standards 

      Glass cuvettes

Cuvettes used only in the visible range, without a lid.

 
 Code Type Optical
pathlength
[mm]
Dimensions
[mm]
 Volume
[ml]
6030-10-10  OG 10 45 x 12.5 x 12.5 3.50
6030-20-10  OG  20 45 x 12.5 x 22.5 7.00
6030-40-10  OG  40 45 x 12.5 x 42.5 14.00
6030-50-10  OG  50 45 x 12.5 x 52.5 17.50

 Special optical glass cuvettes

Cuvettes delivered with lids used up to 320 nm.

 
 Code Type Optical
pathlength
[mm]
Dimensions
[
mm]
 Volume
[ml]
100-1-20  OS 1 45 x 12.5 x 3.5 0.35
 100-2-20  OS  2 45 x 12.5 x 4.5 0.70
  100-5-20  OS  5 45 x 12.5 x 7.5 1.75
  100-10-20  OS 10 45 x 12.5 x 12.5 3.50
 100-20-20 OS 20 45 x 12.5 x 22.5 7.00
 100-40-20 OS 40 45 x 12.5 x 42.5 14.00
 100-50-20 OS 50 45 x 12.5 x 52.5 17.50
 100-100-20 OS 100 45 x 12.5 x 102.5 35.00

Cuvettes delivered with stoppers used up to 320 nm.

 Code Type Optical
pathlength
[mm]
Dimensions
[mm]
 Volume
[ml]
110-5-20  OS 5 46 x 12.5 x 7.5 1.75
110-10-20  OS 10 46 x 12.5 x 12.5 3.50
110-20-20  OS 20 45 x 12.5 x 22.5 7.00
110-40-20  OS 40 45 x 12.5 x 42.5 14.00
110-50-20 OS 50 46 x 12.5 x 52.5 17.50

  Quartz cuvettes

Cuvettes delivered with lids used in the UV-Vis range.

 
 Code Type Optical
pathlength
[mm]
Dimensions
[mm]
 Volume
[ml]
100-1-40  QS 1 45 x 12.5 x 3.5 0.35
100-2-40  QS 2 45 x 12.5 x 4.5 0.70
100-5-40  QS 5 45 x 12.5 x 7.5 1.75
100-10-40  QS 10 45 x 12.5 x 12.5 3.50
100-20-40 QS 20 45 x 12.5 x 22.5 7.00
100-40-40 QS 40 45 x 12.5 x 42.5 14.00
100-50-40 QS 50 45 x 12.5 x 52.5 17.50
 100-100-40 QS 100  45 x 12.5 x 102.5 35.00

Cuvettes with stoppers used in the UV-Vis range.

 Code Type Optical
pathlength
[mm]
Dimensions
[mm]
 Volume
[ml]
110-5-40  QS 5 46 x 12.5 x 7.5 1.75
110-10-40  QS 10 46 x 12.5 x 12.5 3.50
110-20-40  QS 20 45 x 12.5 x 22.5 7.00
110-40-40  QS 40 45 x 12.5 x 42.5 14.00
110-50-40 OS 50 46 x 12.5 x 52.5 17.50

   Semi-micro and micro cuvettes

Tightened cuvettes with PTFE lids.

 
 Code Type Optical
pathlength
[mm]
Dimensions
[mm]
 Volume
[ml]
104-10-20 OS 10 45 x 12.5 x 12.5 1.40
104-10-40 QS 10 45 x 12.5 x 12.5 1.40
104-002-10-20 OS 10  45 x 12.5 x 12.5 0.70
104-002-10-40 QS 10  45 x 12.5 x 12.5 0.70
104002B-10-20 OS 10 45 x 12.5 x 12.5 0.70
104002B-10-40 QS 10 45 x 12.5 x 12,5 0.70
105-10-40 QS 10 25 x 12.5 x 12.5 0.30
 105-B-10-40 QS 10 25 x 12.5 x 12,5 0.30

TrayCell – ultra-mikro quartz cuvettes

Ultra-micro cuvette with fiber optic, the available wavelength range is 190-1100 nm.

 Code Type Optical
pathlength
[mm]
Dimensions
[mm]
 Volume
[µl]
105800-A3-V1-46 QS 0.2 68.5 x 75 x 80 0.7 – 4
1 3 – 5
105810-A3-V1-46 QS 0.2 53 x 59.5 x 64.5 0.7 – 4
1 3 – 5

For the TrayCell cuvettes it is required to specify the centre of the light path height of the spectrophotometer. For the JASCO spectrophotometers this is 15 mm.

The light path length and the required sample volume depend on the removable cap.

Cuvettes for magnetic stirrers

Cuvettes with special bottoms designed for holding the magnetic stirring element, with PTFE lids or stoppers.
 Code Type Optical
pathlength
[mm]
Dimensions
[mm]
 Volume
[ml]
109-000-10-40 QS 10 45 x 12.5 x 12.5 3.50
119-10-40 QS 10 49.5 x 12.5 x 12.5 3.50
109-004-10-40 QS 10 45 x 12.5 x 12.5 1.50
119-004-10-40 QS 10  49.5 x 12.5 x 12.5 1.50

Fluorescence cuvettes

Cuvette with PTFE-lids and stoppers for fluorescence measurements (111).
 Code Type Optical
pathlength
[mm]
Dimensions
[mm]
 Volume
[ml]
101-10-20 OS 10 x 10 45 x 12.5 x 12.5 3.50
101-10-40 QS 10 x 10 45 x 12.5 x 12.5 3.50
111-10-40 QS 10 x 10  46 x 12.5 x 12.5 3.50
104F-10-40 QS 10 x 4   45 x 12.5 x 12.5 1.40
104002F-10-40 QS 10 x 2  45 x 12.5 x 12.5 0.70
109000F-10-40 QS 10 x 10 45 x 12.5 x 12.5 3.50
 111-061-40 QS  triangular 46 x 12.4 x 12.4 1.75

Cylindrical cuvettes

2-port cylindrical cuvettes.

 Code Type Optical
pathlength
[mm]
Dimensions
[mm]
 Volume
[ml]
121-0.10-40 QS 0.1 22 x 20 0.16
121-0.20-40 QS 0.2 22 x 20 0.17
121-0.50-40 QS 0.5 22 x 20 0.21
121-1-40 QS 1 22 x 20 0.28

One-port cylindrical cuvettes and 2-port cylindrical cuvettes over 50 mm light path length.

 Code Type Optical
pathlength
[mm]
Dimensions
[mm]
 Volume
[ml]
120-000-1-40 QS 1 22 x 3.5 0.28
120-000-2-40 QS 2 22 x 4.5 0.56
120-5-40 QS 5 22 x 7.5 1.40
120-10-40 QS 10 22 x 12.5 2.80
120-20-40 QS 20 22 x 22.5 5.60
120-50-40 QS 50 22 x 52.5 14.00
120-100-40 QS 100 22 x 102.5 28.00

Water thermostated cuvettes with water jacket.

 Code Type Optical
pathlength
[mm]
Dimensions
[mm]
 Volume
[ml]
165-0.10-40 QS 0.1 22 x 30 0.10
165-0.50-40 QS 0.5 22 x 30 0.14
165-1-40 QS 1 22 x 30 0.16
165-10-40 QS 10 22 x 12.5 0.80

Flow-through cuvettes

Flow-through cuvettes with inlet and outlet tubes.

 Code Type Optical
pathlength
[mm]
Dimensions
[mm]
 Volume
[ml]
170-700-1-40 QS 1 35 x 12.5 x 12.5 0.062
170-700-2-40 QS 2 35 x 12.5 x 12.5 0.124
1767005-15-40 QS 5 35 x 12.5 x 12.5 0.195
1767001510-40 QS 10 35 x 12.5 x 12.5 0.390
1767001550-40 QS 50 35 x 12.5 x 52.5 1.950
For the 176 code cuvettes it is required to specify the centre of the light path height of the spectrophotometer. For the JASCO spectrophotometers this is 15 mm.

Demountable cuvettes

Rectangular, demountable cuvettes with short light path length.
 Code Type Optical
pathlength
[mm]
Dimensions
[mm]
 Volume
[µl]
106-001-40 QS 0.01 45 x 12.5 2.6
106-010-40 QS 0.1 45 x 12.5 26
106-020-40 QS 0.2 45 x 12.5 52
106-050-40 QS 0.5 45 x 12.5 130
013-000-71 Cuvette holder; dimensions: 45 x 12.5 x 12.5 mm

Calibration standards

Calibration standards with the appropriate certificates.

Type Test Material Wavelength[nm]
666-F1 wavelength Holmium-oxid 279; 361; 453; 536; 638
666-F2 absorbance Neutral Density Glass 440; 465; 546,1; 590; 635
666-F3 absorbance Neutral Density Glass 440; 465; 546,1; 590; 635
666-F4 absorbance Neutral Density Glass 440; 465; 546,1; 590; 635
666-F7 absorbance
wavelength
Didymium Glass Filter 270; 280; 297; 320; 340
329; 472; 512; 681; 875
666.S000 wavelength
absorbance
Calibration standard set: contains the F0, 666-F1, 666-F2, 666-F3 and 666-F4 calibration standards.

For further Hellma cuvettes, standards, light fiber accessories ask for our free catalog!

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