HPLC parts

Sample Filtration image

Sample Filtration

The membrane filters produced by the Spanish Teknokroma company feature good quality, high purity and excellent batch-to-batch reproducibility. In the range of products offered by the company there can also be found syringe filters as well as membrane filters used in the analytical practice and they are recommended for sample and chromatographic eluent filtration, as well.

Syringe filters

In the polypropylene house of the syringe filters a membrane filter with 13 or 25 mm is fitted. For an easy identification, the type of the membrane is indicated by the color of the plastic filter body. Conventional glass (with luer plug) or plastic syringes can be fitted to the filters. The Teknokroma syringe filters are available in resealable plastic boxes, 100 pieces/box.

Specifications:

  • dead volume: 28 μl for 13 mm diameter filter
  • dead volume: 100 μl for 25mm diameter filter

Recommended sample volume

  • for 13 mm diameter filter maximum 10 ml
  • for 25 mm diameter filter maximum 100 ml
  • Pore size: 0.20 μm, 0.45 μm

Low-volume syringe filters

For the filtration of the sample volumes of less than 1 ml, the use of low-volume syringe filters are recommended. In the polypropylene house a 4 mm (Nylon, PTFE, PVDF) membrane filter is fitted. A conventional glass (with luer plug) or plastic syringe can be fitted to the filter.

Membrane types and their characteristics:

Nylon 66

  • hydrophilic membrane
  • filtration rate: fast
  • strong protein binding capacity
  • solvent compatibility: “weak” organic solvents (alcohols, acetonitrile), aqueous solutions, base solutions, THF
  • not recommended for: strong acids, halogenated hydrocarbons
  • use: for general filtration and filtration of HPLC and dissolution samples

PTFE (poly(tetrafluoroethylene))

  • hydrophobic membrane
  • filtration rate: medium
  • low dissolution, best solvent resistance
  • solvent compatibility: “strong” organic solvents, strong acids, strong alkalis, chlorinated hydrocarbons
  • not recommended for: aqueous solutions
  • use: for organic solvents, general filtration and filtration of HPLC samples

M. E. Cellulose (mixed cellulose ester, nitrate and acetate)

  • hydrophilic membrane
  • filtration rate: medium
  • solvent compatibility: aqueous solutions, ”weak” base solutions, hydrocarbons
  • not recommended for: ”strong” organic solvents, strong acids, strong bases
  • used for the filtration of aqueous solutions, biological samples and HPLC and dissolution samples

PVDF (poly(vinylidene fluoride))

  • hydrophilic membrane
  • filtration rate: medium
  • low dissolution
  • weak protein binding capacity
  • solvent compatibility: aqueous and organic solutions, weak base solutions, hydrocarbons, halogenated hydrocarbons
  • not recommended for: strong acids, strong bases, ketones, ethers
  • used for the filtration of aqueous solutions, proteins, peptides, biological samples, HPLC and dissolution samples

 

Code Pore size[μm] Diameter[mm] Color
Membrane: Nylon 66
TR-200100 0.45 25 green
TR-200101 0.20 25 light green
TR-200500 0.45 13 green
TR-200501 0.20 13 light green
Membrane: PTFE
TR-200102 0.45 25 blue
TR-200103 0.20 25 light blue
TR-200502 0.45 13 blue
TR-200503 0.20 13 light blue
Membrane: M. E. Cellulose
TR-200104 0.45 25 yellow
TR-200105 0.20 25 light yellow
TR-200504 0.45 13 yellow
TR-200505 0.20 13 light yellow
Membrane: PVDF
TR-200106 0.45 25 red
TR-200107 0.20 25 light red
TR-200506 0.45 13 red
TR-200507 0.20 13 light red

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